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產品目錄
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ANNEXIN V-FITC/PI凋亡檢測試劑盒 CA1020
物品單位 價格 品牌
20T 680 solarbio
  • 產地:北京
  • 貨號:CA1020
  • 發布日期: 2019-12-18
  • 更新日期: 2020-03-05
產品詳細說明
產地 北京
品牌 solarbio
貨號 CA1020
用途 細胞凋亡是細胞的基本特征之一,在機體的胚胎發育、組織修復、內環境的穩定等方面都起到十分重要的作用。
產品規格 20T/50T/100T
CAS編號
純度 現詢客服%
是否進口

ANNEXIN V- FITC/PI 凋亡檢測試劑盒.








產品說明:

細胞凋亡是細胞的基本特征之一,在機體的胚胎發育、組織修復、內環境的穩定等方面都起到 十分重要的作用。Annexin V 是一種分子量為 35.8 KD 的 Ca2+依賴性磷脂結合蛋白,能與細胞凋亡 過程中翻轉到膜外的磷脂酰絲氨酸(Phosphatidylserine, PS)高親和力特異結合。FITC-Annexin V 結 合到凋亡細胞后,在藍色光的激發下,發出綠色熒光,區分出凋亡細胞與正常細胞。碘化丙啶 (Propidium iodide,PI)是一種核酸染料,它不能穿透完整細胞膜,但對凋亡晚期細胞和死細胞的 破損細胞膜能夠穿透,并使細胞核紅染。將 FITC-Annexin V 與PI 匹配使用,可以將凋亡早期的細 胞和晚期的細胞區分開來。 :

操作步驟
1、 用 27ml 的去離子水稀釋 3ml Binding Buffer(10×)至 30ml,每次用 3ml。
2、 收集細胞(1×106 個/次),然后用冷的 PBS 洗滌。(對于貼壁細胞,先用胰酶消化,再用 PBS 洗 滌)。
3、 用 1ml 1×的 Binding Buffer 懸浮細胞,300×g 離心 10min,棄上清。
4、 用 1ml 1×的 Binding Buffer 重懸細胞,使細胞的密度達到 1×106 個/ml。
5、 每管加入 100μL 細胞(1×105 個)。
6、 再向管中加入 5μL Annexin V-FITC。
7、 室溫,避光,輕輕地混勻,10min。
8、 加入 5μL PI,室溫,避光,孵育 5min。
9、 加入 PBS 至 500μL,輕輕混勻。
10、在 1 小時內用流式細胞儀檢測。

注意事項
1、旋帽離心管裝的試劑在開蓋前請短暫離心,將蓋內壁上的液體甩至管底,避免開蓋時液體灑落。
2、Annexin V-FITC 和 Propidium iodide 是光敏物質,在保存與操作時請注意避光。
3、Propidium iodide(PI)能通過皮膚吸收,對眼睛有刺激作用。
4、在細胞洗滌的*一步,請盡量將上清棄凈,以免 PBS 殘留影響實驗結果。 產品內容: CA1020-20 CA1020-50 CA1020-100 保存 Annexin V-FITC 100μl 250μl 500μl 4°C,避光,不要冷凍 Propidium iodide(PI) 100μl 250μl 500μl 4°C,避光,不要冷凍 Binding Buffer(10×) 6ml 15ml 30ml 4°C 保存,長時間-20°C
5、PI 染色時間過長有可能造成檢測的凋亡率偏高,建議首先進行 Annexin V-FITC 染色,上機前 5 分鐘再加入PI 染色。
6、整個操作過程動作要盡量輕柔,勿用力吹打細胞,盡量在 4℃下操作。
7、反應完畢后請盡快檢測,因為細胞凋亡是一個動態的過程,反應 1 小時后熒光強度就開始衰變。
8、成功的檢測凋亡受以下幾種因素的影響,如細胞類型、細胞膜上 PS 的密度、發生凋亡時 PS 翻 轉的比例、誘導細胞凋亡的方法、所用試劑、誘導凋亡的時間等,把這些影響因素進行優化對實驗 成功是非常必要的。 此試劑盒僅供科研使用。


相關產品:

G3680    細胞凋亡-Hoechst染色試劑盒

CA1120   細胞凋亡-Hoechst染色試劑盒

T2190    TUNEL細胞凋亡檢測試劑盒

CA1030   Annexin V PE/7-AAD 凋亡檢測試劑盒

CA1040   AnnexinV Alexa Fluor488/PI 凋亡檢測試劑盒

CA1050   AnnexinV Alexa Fluor647/PI 凋亡檢測試劑盒

BC3810   Caspase-1活性測定試劑盒

C6700    CCCP 細胞凋亡誘導劑(50mM


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《CRISPR/Cas9-mediated gene knockout of ARID1A promotes primary progesterone resistance by downregulating progesterone receptor B in endometrial cancer cells.》 作者: Wang, Haizhen1; Tang, Zhenghua1; Li, Ting1; Liu, Menglu1; Li, Yong2; Xing, Baoling 期刊:Oncol. Res. 影響因子:4.634 PMID:31072420
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