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中性蛋白酶II;分散酶II 分散酶2 中性蛋白酶2;D6430
物品單位 價格 品牌
520 Solarbio
  • 產地:北京
  • 貨號:D6430
  • cas:42613-33-2
  • 發布日期: 2019-12-19
  • 更新日期: 2019-12-19
產地 北京
品牌 Solarbio
貨號 D6430
用途范圍 The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml.
英文名稱 Dispase II
純度 0.5 units/mg solid%
CAS編號 42613-33-2
規格 100mg
保質期 1年
溶解性Solubility:10mM NaAc(pH 7.5)and 5mM CaAc
別名分散酶II 分散酶2 中性蛋白酶2
英文名稱Dispase II
酶活/效價0.5 units/mg solid

Analysis Note
The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by divalent cations including Ca2+ , Mg2+, Mn2+ and Fe2+.
Preparation Note
A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.
Physical form
lyophilized powder containing calcium acetate and milk sugar
The enzyme has been used in developing a protocol for ex vivo culture of mouse embryonic mammary buds.[4] It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart.[5] It has also been used for the isolation of dental pulp stem cells (DPSCs) by enzymatic hydrolysis. These cells have been compared with DPSCs isolated by explant method to analyse their stem cell and differentiation properties.[1]
Dispase II has been used for Fluorescence-Activated Cell Sorting (FACS).[7] It has also been used for separating visceral yolk sac layers.[2]
Biochem/physiol Actions

Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.[3] Dispase II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+ activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity.[6] The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.

Solubility:10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble


《Establishment of an immortalized intestinal epithelial cell line from tree shrews by lentivirus-mediated hTERT gene transduction》 作者:Bowen Yin,Qingkai Song,Lingxia Chen,Xiaofei Li,Yuanyuan Han,Xuan Wang,Jiejie Dai,Xiaomei Sun 期刊:Cytotechnology 影響因子:1.672 PMID:30603916

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